Hi MBF Team,
 
I was creating a test program to submit a sequence to BioHPC QBLAST:
 
using System;
using System.Collections.Generic;
using System.Threading;
using System.Web;
using MBF;
using MBF.IO.Fasta;
using MBF.Web;
using MBF.Web.Blast;
using MBF.Web.BioHPC;
using System.Diagnostics;
 
namespace PrototypePivotGenomeAnnotationPipeline
{

class Program
{
    static void Main(string[] args)
    {
        FastaParser parser = new FastaParser();

        // A file with one sequence for debugging.
        IList<ISequence> proteinList = parser.Parse(args[0]);

        ConfigParameters configParams = new ConfigParameters();
        configParams.UseBrowserProxy = true;

        BlastParameters searchParams = new BlastParameters();
        searchParams.Add("Program", "blastp");
        searchParams.Add("Database", "nr");
        searchParams.Add("Expect", "1e-20");
        searchParams.Add("Email", "t-vinfor@microsoft.com");

        BioHPCBlastHandler blastService = new BioHPCBlastHandler();

        blastService.Configuration = configParams;

        string jobId;
        jobId = blastService.SubmitRequest(proteinList, searchParams);
        Thread.Sleep(20000);
        ServiceRequestInformation info = blastService.GetRequestStatus(jobId);

        string result = blastService.GetResult(jobId, searchParams);

        Debug.WriteLine(result);
    }

 

}

}
 
And got the following Exception:
 
System.Exception was unhandled
Message=The Email parameter is required.Parameter \"EMAIL\" is not valid for BioHPC QBLAST.
Source=MBF.WebServiceHandlers
StackTrace:

   at MBF.Web.Blast.BioHPCBlastHandler.SubmitRequest(IList`1 sequences, BlastParameters parameters) in C:\Users\t-vinfor\Desktop\mbf-60502\MBF\MBF.WebServiceHandlers\BioHPC\Blast\BioHPCBlastHandler.cs:line 438
   at PrototypePivotGenomeAnnotationPipeline.Program.Main(String[] args) in C:\Users\t-vinfor\documents\visual studio 2010\Projects\PrototypePivotGenomeAnnotationPipeline\BioHPCBLASTp\Program.cs:line 37
   at System.AppDomain._nExecuteAssembly(RuntimeAssembly assembly, String[] args)
   at System.AppDomain.ExecuteAssembly(String assemblyFile, Evidence assemblySecurity, String[] args)
   at Microsoft.VisualStudio.HostingProcess.HostProc.RunUsersAssembly()
   at System.Threading.ThreadHelper.ThreadStart_Context(Object state)
   at System.Threading.ExecutionContext.Run(ExecutionContext executionContext, ContextCallback callback, Object state, Boolean ignoreSyncCtx)
   at System.Threading.ExecutionContext.Run(ExecutionContext executionContext, ContextCallback callback, Object state)
   at System.Threading.ThreadHelper.ThreadStart()

InnerException:
 
When I remove "searchParams.Add("Email", "t-vinfor@microsoft.com");" I get the following Exception:
 
System.Exception was unhandled
Message=The Email parameter is required.
Source=MBF.WebServiceHandlers
StackTrace:

   at MBF.Web.Blast.BioHPCBlastHandler.SubmitRequest(IList`1 sequences, BlastParameters parameters) in C:\Users\t-vinfor\Desktop\mbf-60502\MBF\MBF.WebServiceHandlers\BioHPC\Blast\BioHPCBlastHandler.cs:line 438
   at PrototypePivotGenomeAnnotationPipeline.Program.Main(String[] args) in C:\Users\t-vinfor\documents\visual studio 2010\Projects\PrototypePivotGenomeAnnotationPipeline\BioHPCBLASTp\Program.cs:line 37
   at System.AppDomain._nExecuteAssembly(RuntimeAssembly assembly, String[] args)
   at System.AppDomain.ExecuteAssembly(String assemblyFile, Evidence assemblySecurity, String[] args)
   at Microsoft.VisualStudio.HostingProcess.HostProc.RunUsersAssembly()
   at System.Threading.ThreadHelper.ThreadStart_Context(Object state)
   at System.Threading.ExecutionContext.Run(ExecutionContext executionContext, ContextCallback callback, Object state, Boolean ignoreSyncCtx)
   at System.Threading.ExecutionContext.Run(ExecutionContext executionContext, ContextCallback callback, Object state)
   at System.Threading.ThreadHelper.ThreadStart()

InnerException:
 
Somewhat circular logic, or is the process to submit a job to BioHPCBlastHandler different than NCBIBlastHandler?
 
Thanks.
 
Vince